p gp  (Cell Signaling Technology Inc)

Journal: Neural Regeneration Research

Article Title: Bromocriptine protects perilesional spinal cord neurons from lipotoxicity after spinal cord injury

doi: 10.4103/1673-5374.385308

Figure Lengend Snippet: BRO alleviates LD accumulation via the ERK1/2/cPLA2 pathway in MD-treated HT22 cells. (A–C) Phosphorylation of ERK1/2 and cPLA2 were detected by western blotting analysis (mean ± SD, three independent experiments, * P < 0.05, *** P < 0.001, **** P < 0.0001, one-way analysis of variance followed by a post hoc Tukey’s test). (D, E) Flow cytometry analysis of MD-treated HT22 cells treated with U0126 stained with BD493 (mean ± SD, three independent experiments, *** P < 0.001, **** P < 0.0001, one-way analysis of variance followed by a post hoc Tukey’s test). BD493: Boron-dipyrromethene 493/503; BRO: bromocriptine; cPLA2: cytosolic phospholipase A2; ERK1/2: extracellular signal-regulated kinases 1/2; FITC: fluorescein isothiocyanate; GSEA: Gene Set Enrichment Analysis; KEGG: Kyoto Encyclopedia of Genes and Genomes; MD: myelin debris; ns: not significant; PCA: principal component analysis.

Article Snippet: The membranes were blocked with 5% bovine serum albumin (Sigma-Aldrich, Cat# A1933) for 1 hour at room temperature and subsequently incubated with rabbit anti-Erk1/2 (1:1000, Abclonal, Cat# A16686, RRID: AB_2770274), rabbit anti-p-Erk1/2 (1:1000, Abclonal, Cat# AP0472), rabbit anti-cPLA2 (1:1000, Abclonal, Cat# A0394, RRID: AB_2757169), rabbit anti-p-cPLA2 (1:1000, Abclonal, Cat# AP0968), and rabbit anti-glyceraldehyde phosphate dehydrogenase (GAPDH; 1:1000, Abclonal, Cat# AC001, RRID: AB_2619673) primary antibodies at 4°C overnight.

Techniques: Western Blot, Flow Cytometry, Staining